This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223-ERBB2
catalog :
23888
citations: 10
Reference
Mitani Y, Ohashi S, Kikuchi O, Nakai Y, Ida T, Mizumoto A, et al. HER2 G776S mutation promotes oncogenic potential in colorectal cancer cells when accompanied by loss of APC function. Sci Rep. 2022;12:9213 pubmed publisher
Johnson A, Wei J, Rosser J, Künkele A, Chang C, Reid A, et al. Rationally Designed Transgene-Encoded Cell-Surface Polypeptide Tag for Multiplexed Programming of CAR T-cell Synthetic Outputs. Cancer Immunol Res. 2021;: pubmed publisher
Al Akhrass H, Conway J, Poulsen A, Paatero I, Kaivola J, Padzik A, et al. A feed-forward loop between SorLA and HER3 determines heregulin response and neratinib resistance. Oncogene. 2021;: pubmed publisher
Schulz D, Zanotelli V, Fischer J, Schapiro D, Engler S, Lun X, et al. Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry. Cell Syst. 2018;6:25-36.e5 pubmed publisher
Yang Y, Xiong D, Pipathsouk A, Weiner O, Wu M. Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning. Dev Cell. 2017;43:507-521.e4 pubmed publisher
Werbin J, Avendaño M, Becker V, Jungmann R, Yin P, Danuser G, et al. Multiplexed Exchange-PAINT imaging reveals ligand-dependent EGFR and Met interactions in the plasma membrane. Sci Rep. 2017;7:12150 pubmed publisher
Carter E, Gopsill J, Gomm J, Jones J, Grose R. A 3D in vitro model of the human breast duct: a method to unravel myoepithelial-luminal interactions in the progression of breast cancer. Breast Cancer Res. 2017;19:50 pubmed publisher
Fowler D, Stewart S, Seredick S, Eisen J, Stankunas K, Washbourne P. A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications. PLoS ONE. 2016;11:e0159277 pubmed publisher
Witkiewicz A, Cox D, Rivadeneira D, Ertel A, Fortina P, Schwartz G, et al. The retinoblastoma tumor suppressor pathway modulates the invasiveness of ErbB2-positive breast cancer. Oncogene. 2014;33:3980-91 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher
product information
Catalog Number :
23888
Product Name :
pDONR223-ERBB2
article :
doi10.1038/nature09627
id3878
pubmed_id21107320
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Donor vector
growth notes :
The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
origin :
37
pi :
alt_names
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2)
geneERBB2
id2064
genbank_ids
mutation
nameERBB2
shRNA_sequence
size3768
species
9606
Homo sapiens
tags
resistance markers :
184
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA