This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223-RPS6KA2
catalog :
23530
citations: 3
Reference
Sundell G, Arnold R, Ali M, Naksukpaiboon P, Orts J, Güntert P, et al. Proteome-wide analysis of phospho-regulated PDZ domain interactions. Mol Syst Biol. 2018;14:e8129 pubmed publisher
Sadaie W, Harada Y, Matsuda M, Aoki K. Quantitative in vivo fluorescence cross-correlation analyses highlight the importance of competitive effects in the regulation of protein-protein interactions. Mol Cell Biol. 2014;34:3272-90 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher
product information
Catalog Number :
23530
Product Name :
pDONR223-RPS6KA2
article :
doi10.1038/nature09627
id3878
pubmed_id21107320
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Donor vector
growth notes :
The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
origin :
37
pi :
alt_names
ribosomal protein S6 kinase, 90kDa, polypeptide 2
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHU-2, MAPKAPK1C, RSK, RSK3, S6K-alpha, S6K-alpha2, p90-RSK3, p90RSK2, pp90RSK3
geneRPS6KA2
id6196
genbank_ids
mutationC108T, A620G,2MM
nameRPS6KA2
shRNA_sequence
size2202
species
9606
Homo sapiens
tags
resistance markers :
184
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA