This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223-CAMK2A
catalog :
23408
citations: 5
Reference
Lu x10d i x107 I, Jiang P, Franz A, Bursztyn Y, Liu F, Plested A. Controlling the interaction between CaMKII and Calmodulin with a photocrosslinking unnatural amino acid. Protein Sci. 2023;32:e4798 pubmed publisher
Ventura E, Iannuzzi C, Pentimalli F, Giordano A, Morrione A. RBL1/p107 Expression Levels Are Modulated by Multiple Signaling Pathways. Cancers (Basel). 2021;13: pubmed publisher
Sim K, Lee Y, Kim H, Cho C, Yi G, Park M, et al. Suppression of CaMKIIβ Inhibits ANO1-Mediated Glioblastoma Progression. Cells. 2020;9: pubmed publisher
Royer L, Herzog J, Kenny K, Tzvetkova B, Cochrane J, Marr M, et al. The Ras-like GTPase Rem2 is a potent inhibitor of calcium/calmodulin-dependent kinase II activity. J Biol Chem. 2018;293:14798-14811 pubmed publisher
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed publisher
product information
Catalog Number :
23408
Product Name :
pDONR223-CAMK2A
article :
doi10.1038/nature09627
id3878
pubmed_id21107320
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Donor vector
growth notes :
The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
origin :
37
pi :
alt_names
calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCAMKA, CaMKIINalpha, CaMKIIalpha, MRD53, MRT63
geneCAMK2A
id815
genbank_ids
mutation
nameCAMK2A
shRNA_sequence
size1437
species
9606
Homo sapiens
tags
resistance markers :
184
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA