This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Tpa1(273-644)
catalog :
23141
citations: 1
Reference
Kim H, Kim H, Kim K, Kim D, Lee S, Yoon J, et al. Crystal structure of Tpa1 from Saccharomyces cerevisiae, a component of the messenger ribonucleoprotein complex. Nucleic Acids Res. 2010;38:2099-110 pubmed publisher
product information
Catalog Number :
23141
Product Name :
Tpa1(273-644)
article :
doi10.1093/nar/gkp1151
id3092
pubmed_id20040577
bacterial resistance :
Ampicillin
cloning :
backbonepET21a(+)
backbone_mutation
backbone_origin
backbone_size5443
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This construct has a mutation causing N565D in the aa sequence. The depositor does know the effect of this mutation. However, it is not part of the active site.
origin :
37
pi :
alt_names
Tpa1 CTD
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYER049W
geneTPA1
id856775
genbank_ids
mutationdeleted amino acids 1-272
nameTpa1 C-terminal domain
shRNA_sequence
size1116
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on backbone
tagHis
resistance markers :
508
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA