This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRR-ERalpha-5Z
catalog :
23061
citations: 2
Reference
Zhang H, Cui Z, Yang B, Fang D, Liu Y, Wang Z. Integrated recombinant gene yeast bioassay and HPLC-MS analysis for detection of low-dose multi-component residue of hormone-like compounds in environment. Sci Total Environ. 2021;773:145569 pubmed publisher
Miller C, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Plasmid. 2010;63:73-8 pubmed publisher
product information
Catalog Number :
23061
Product Name :
pRR-ERalpha-5Z
article :
doi10.1016/j.plasmid.2009.11.003
id3077
pubmed_id19962400
bacterial resistance :
Ampicillin
cloning :
backbonepRW95-3
backbone_mutation
backbone_originPMID: 8800671
backbone_size8700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
There is a M517T mutation in the ESR1 sequence. The mutation should not have any effect on protein function and this construct worked the same when assessed in yeast signaling assays and estrogen signaling.
origin :
37
pi :
alt_names
estrogen receptor alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3SnaBI
cloning_site_5SnaBI
promoter
sequencing_primer_3
sequencing_primer_5GAL1
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesER, ESR, ESRA, ESTRR, Era, NR3A1
geneESR1
id2099
genbank_ids
mutation
nameERalpha
shRNA_sequence
size3000
species
9606
Homo sapiens
tags
plasmid copy :
The cDNA used to create this plasmid was obtained from Keith R. Yamamoto.
resistance markers :
660
tags :
High Copy
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA