pRR-AR-5Z | Addgene 23058 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRR-AR-5Z

catalog :

23058

citations: 2

Reference
Zhang H, Cui Z, Yang B, Fang D, Liu Y, Wang Z. Integrated recombinant gene yeast bioassay and HPLC-MS analysis for detection of low-dose multi-component residue of hormone-like compounds in environment. Sci Total Environ. 2021;773:145569 pubmed publisher
Miller C, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Plasmid. 2010;63:73-8 pubmed publisher

product information

Catalog Number :

23058

Product Name :

pRR-AR-5Z

article :

doi	10.1016/j.plasmid.2009.11.003
id	3077
pubmed_id	19962400

bacterial resistance :

Ampicillin

cloning :

backbone

pRW95-3

backbone_mutation

backbone_origin

PMID: 8800671

backbone_size

8700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

Amino acid G8 is missing, BLAST shows different number of Qs in sequence. Please note that this construct was tested by the depositing lab. Dr Miller transformed yeast strain W303a with Addgene's plasmid and with his stock of the same plasmid. He also streaked out yeast that were previously transformed with this plasmid and validated for signaling of the lacZ reporter gene in response to testosterone. The plasmids transformed well and conferred reporter gene signaling in the transformants when exposed to 25 nM testosterone. This bioassay indicates that the human androgen receptor DNA sequence produces a functional protein.

origin :

pi :

alt_names

androgen receptor

cloning

clone_method	Restriction Enzyme
cloning_site_3	SnaBI
cloning_site_5	SnaBI
promoter
sequencing_primer_3
sequencing_primer_5	GPDpro-F
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
gene	AR
id	367

genbank_ids

mutation

name

shRNA_sequence

size

3000

species

9606

Homo sapiens