This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGL3-p27 T187A Luc
catalog :
23048
citations: 1
Reference
Zhang G, Safran M, Wei W, Sorensen E, Lassota P, Zhelev N, et al. Bioluminescent imaging of Cdk2 inhibition in vivo. Nat Med. 2004;10:643-8 pubmed
product information
Catalog Number :
23048
Product Name :
pGL3-p27 T187A Luc
article :
doi10.1038/nm1047
id3076
pubmed_id15122251
bacterial resistance :
Ampicillin
cloning :
backbonepGL3-control
backbone_mutation
backbone_originPromega
backbone_size5223
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth temp :
DH5alpha
origin :
37
pi :
alt_names
kip1
cloning
clone_methodRestriction Enzyme
cloning_site_3Nco I
cloning_site_5Hind III
promoter
sequencing_primer_3
sequencing_primer_5GLprimer2 (Promega)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDKN4, KIP1, MEN1B, MEN4, P27KIP1
geneCDKN1B
id1027
genbank_ids
U10906
mutationT187A: mutation at codon 187 changing from T (ACG) to A (GCG), additionally 3 silent mutations introducing a BamH I site at codons 182/183/184 (GGT TCT GTG) to (GGA TCC GTC)
namep27
shRNA_sequence
size607
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagLuciferase
resistance markers :
238
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA