This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCI-neo-hApg5(K130R)-HA
catalog :
22949
citations: 3
Reference
Shroff A, Reddy K. Autophagy gene ATG5 knockdown upregulates apoptotic cell death during Candida albicans infection in human vaginal epithelial cells. Am J Reprod Immunol. 2018;80:e13056 pubmed publisher
Schnöder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, et al. Deficiency of Neuronal p38α MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1. J Biol Chem. 2016;291:2067-79 pubmed publisher
Mizushima N, Sugita H, Yoshimori T, Ohsumi Y. A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J Biol Chem. 1998;273:33889-92 pubmed
product information
Catalog Number :
22949
Product Name :
pCI-neo-hApg5(K130R)-HA
article :
doi10.1074/jbc.273.51.33889
id3047
pubmed_id9852036
bacterial resistance :
Ampicillin
cloning :
backbonepCI-neo
backbone_mutation
backbone_originPromega
backbone_size5472
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
Atg5
Apg5
cloning
clone_methodRestriction Enzyme
cloning_site_3SmaI
cloning_site_5SmaI
promoter
sequencing_primer_3T7
sequencing_primer_5T3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAPG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5
geneATG5
id9474
genbank_ids
mutation130 Lysine to Arginine
nameHomo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae)
shRNA_sequence
size830
species
9606
Homo sapiens
tags
locationC terminal on insert
tag3xHA
resistance markers :
644
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA