This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti6/V5-p53_R175H
catalog :
22936
citations: 9
Reference
Haagsma J, Kolendowski B, Buensuceso A, Valdes Y, DiMattia G, Shepherd T. Gain-of-function p53R175H blocks apoptosis in a precursor model of ovarian high-grade serous carcinoma. Sci Rep. 2023;13:11424 pubmed publisher
Menendez D, Anand J, Murphy C, Bell W, Fu J, Slepushkina N, et al. Etoposide-induced DNA damage is increased in p53 mutants: identification of ATR and other genes that influence effects of p53 mutations on Top2-induced cytotoxicity. Oncotarget. 2022;13:332-346 pubmed publisher
Dash S, Yoder S, Mesa T, Smith A, Cen L, Eschrich S, et al. Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells. Sci Rep. 2021;11:14334 pubmed publisher
Lakshmanan I, Chaudhary S, Vengoji R, Seshacharyulu P, Rachagani S, Carmicheal J, et al. ST6GalNAc-I promotes lung cancer metastasis by altering MUC5AC sialylation. Mol Oncol. 2021;15:1866-1881 pubmed publisher
Sa J, Hwang J, Cho Y, Ryu J, Choi J, Jeong S, et al. Pharmacogenomic analysis of patient-derived tumor cells in gynecologic cancers. Genome Biol. 2019;20:253 pubmed publisher
Hashimoto A, Hashimoto S, Sugino H, Yoshikawa A, Onodera Y, Handa H, et al. ZEB1 induces EPB41L5 in the cancer mesenchymal program that drives ARF6-based invasion, metastasis and drug resistance. Oncogenesis. 2016;5:e259 pubmed publisher
Hashimoto A, Oikawa T, Hashimoto S, Sugino H, Yoshikawa A, Otsuka Y, et al. P53- and mevalonate pathway-driven malignancies require Arf6 for metastasis and drug resistance. J Cell Biol. 2016;213:81-95 pubmed publisher
George S, Milea A, Sowamber R, Chehade R, Tone A, Shaw P. Loss of LKB1 and p53 synergizes to alter fallopian tube epithelial phenotype and high-grade serous tumorigenesis. Oncogene. 2016;35:59-68 pubmed publisher
Junk D, Vrba L, Watts G, Oshiro M, Martinez J, Futscher B. Different mutant/wild-type p53 combinations cause a spectrum of increased invasive potential in nonmalignant immortalized human mammary epithelial cells. Neoplasia. 2008;10:450-61 pubmed
product information
Catalog Number :
22936
Product Name :
pLenti6/V5-p53_R175H
article :
doi
id3042
pubmed_id18472962
bacterial resistance :
Ampicillin
cloning :
backbonepLenti6/V5-D-Topo
backbone_mutation
backbone_origin
backbone_size6963
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth temp :
Try using 50 ug/ml ampicillin if the bacteria do not grow well at 100 ug/ml.
origin :
37
pi :
alt_names
TP53
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutationR175H Mutant
nametumor protein 53
shRNA_sequence
size1179
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagV5
resistance markers :
658
tags :
Unknown
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA