pcDNA3-Flag-RPA2 | Addgene 22893 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3-Flag-RPA2

catalog :

22893

citations: 2

Reference
Inano S, Sato K, Katsuki Y, Kobayashi W, Tanaka H, Nakajima K, et al. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol Cell. 2017;66:622-634.e8 pubmed publisher
Unsal Kaçmaz K, Chastain P, Qu P, Minoo P, Cordeiro Stone M, Sancar A, et al. The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement. Mol Cell Biol. 2007;27:3131-42 pubmed

product information

Catalog Number :

22893

Product Name :

pcDNA3-Flag-RPA2

article :

doi	10.1128/MCB.02190-06
id	3035
pubmed_id	17296725

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

There is one point mutation in the insert, but the protein expressed with this construct forms complexes with the other subunits of the RPA heterotrimer, binds other known interacting proteins (XPA, Tipin), and shows an enrichment in the chromatin fraction of cells treated with replication stress-inducing agents. So by these criteria the construct appears to be WT.

origin :

pi :

alt_names

RPA34

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	REPA2, RP-A p32, RP-A p34, RPA32
gene	RPA2
id	6118

genbank_ids

mutation

name

RPA2

shRNA_sequence

size

810

species

9606

Homo sapiens