This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HPRT
catalog :
22884
citations: 1
Reference
Zwaka T, Thomson J. Homologous recombination in human embryonic stem cells. Nat Biotechnol. 2003;21:319-21 pubmed
product information
Catalog Number :
22884
Product Name :
HPRT
article :
doi10.1038/nbt788
id2598
pubmed_id12577066
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript SK (-)
backbone_mutation
backbone_originStratagene
backbone_size9000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Cre/Lox
Other
homologous recombination
growth notes :
Note that very little of the insert has been sequenced by Addgene. Please be sure to do your own sequencing of HPRT before using this plasmid.
origin :
37
pi :
alt_names
HPRT; HGPRT; HPRT1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI and NotI
cloning_site_5SalI and XmaI
promoter
sequencing_primer_3pLac
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHGPRT, HPRT
geneHPRT1
id3251
genbank_ids
NC_000023
mutationPGK, NeoR, and BGH polyA are between the two genomic sequences (one section is 9.1kb and one is 2.5kb).
namehypoxanthine phosphoribosyltransferase-1
shRNA_sequence
size11900
species
9606
Homo sapiens
tags
resistance markers :
523
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA