myc-Orai1 E190A pHAGE | Addgene 22753 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

myc-Orai1 E190A pHAGE

catalog :

22753

citations: 1

Reference
Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan P. Orai1 is an essential pore subunit of the CRAC channel. Nature. 2006;443:230-3 pubmed

product information

Catalog Number :

22753

Product Name :

myc-Orai1 E190A pHAGE

article :

doi	10.1038/nature05122
id	2324
pubmed_id	16921383

bacterial resistance :

Ampicillin

cloning :

backbone

pHAGE

backbone_mutation

backbone_origin

Gift of Richard Mulligan

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

For lentiviral transduction, N-terminally myc-tagged versions of Orai1 were directly cloned NotI-BamHI into the bicistronic pHAGE-UBC-eGFP-IRES-eGFP vector (R. Mulligan, Harvard Medical School), replacing the first eGFP with myc-Orai1. Lab Plasmid # SF214. The Author's sequence includes the ORF plus 60 nt of vector boundary 3' and 5'.

origin :

pi :

alt_names

Orai1

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NotI
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CRACM1, IMD9, ORAT1, TAM2, TMEM142A
gene	ORAI1
id	84876

genbank_ids

mutation

E190A

name

Orai1 E190A

shRNA_sequence

size

1000

species

9606

Homo sapiens