HA-EglN3 H196A-pcDNA3 | Addgene 22717 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

HA-EglN3 H196A-pcDNA3

catalog :

22717

citations: 3

Reference
Schoepflin Z, Silagi E, Shapiro I, Risbud M. PHD3 is a transcriptional coactivator of HIF-1? in nucleus pulposus cells independent of the PKM2-JMJD5 axis. FASEB J. 2017;31:3831-3847 pubmed publisher
Heir P, Srikumar T, Bikopoulos G, Bunda S, Poon B, Lee J, et al. Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling. J Biol Chem. 2016;291:7357-72 pubmed publisher
Lee S, Nakamura E, Yang H, Wei W, Linggi M, Sajan M, et al. Neuronal apoptosis linked to EglN3 prolyl hydroxylase and familial pheochromocytoma genes: developmental culling and cancer. Cancer Cell. 2005;8:155-67 pubmed

product information

Catalog Number :

22717

Product Name :

HA-EglN3 H196A-pcDNA3

article :

doi	10.1016/j.ccr.2005.06.015
id	2221
pubmed_id	16098468

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5445

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

this mutant version of HA-EglN3 H196A-pcDNA3 can not be distinguished from its parental wt plasmid by restriction digest

growth temp :

DH5alpha

origin :

pi :

alt_names

PHD3

prolyl Hydroxylase 3

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoR I
cloning_site_5	Bgl II
promoter
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	HIFP4H3, HIFPH3, PHD3
gene	EGLN3
id	112399

genbank_ids

BC010992

mutation

mutant cDNA: point mutation at codon 196 was inserted to generate mutation H196A

name

Egg laying Nine - 3

shRNA_sequence

size

774

species

9606

Homo sapiens