This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pmMyt1
catalog :
22713
citations: 2
Reference |
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Islam M, Smith D, Niu W, Fang S, Iqbal N, Sun G, et al. Enhancer Analysis Unveils Genetic Interactions between TLX and SOX2 in Neural Stem Cells and In Vivo Reprogramming. Stem Cell Reports. 2015;5:805-15 pubmed
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Kim J, Armstrong R, v Agoston D, Robinsky A, Wiese C, Nagle J, et al. Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. J Neurosci Res. 1997;50:272-90 pubmed
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product information
Catalog Number :
22713
Product Name :
pmMyt1
article :
doi | 10.1002/(SICI)1097-4547(19971015)50:2<272::AID-JNR16>3.0.CO;2-A |
id | 2965 |
pubmed_id | 9373037 |
bacterial resistance :
Ampicillin
cloning :
backbone | pCRII | ||
backbone_mutation | |||
backbone_origin | Invitrogen | ||
backbone_size | 3948 | ||
promoter | |||
sequencing_primer_3 | |||
sequencing_primer_5 | |||
vector_types |
|
growth notes :
The murine Myt1 cDNA (6 zinc fingers) had 3 base mutations within the coding region (bp 806, 837 and 4344). These sites were corrected using Stratagene QuickChang Site-Directed mutagenesis. The Myt1 was sequenced after the mutagenesis and found to be correct. There is still one mutation in the 5' UTR at bp 529 (START codon is bp 532)
growth temp :
Normal growth conditions
origin :
37
pi :
|
plasmid copy :
DNA cloned in Hudson laboratory
resistance markers :
632 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments