This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pmMyt1
catalog :
22713
citations: 2
Reference
Islam M, Smith D, Niu W, Fang S, Iqbal N, Sun G, et al. Enhancer Analysis Unveils Genetic Interactions between TLX and SOX2 in Neural Stem Cells and In Vivo Reprogramming. Stem Cell Reports. 2015;5:805-15 pubmed
Kim J, Armstrong R, v Agoston D, Robinsky A, Wiese C, Nagle J, et al. Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. J Neurosci Res. 1997;50:272-90 pubmed
product information
Catalog Number :
22713
Product Name :
pmMyt1
article :
doi10.1002/(SICI)1097-4547(19971015)50:2<272::AID-JNR16>3.0.CO;2-A
id2965
pubmed_id9373037
bacterial resistance :
Ampicillin
cloning :
backbonepCRII
backbone_mutation
backbone_originInvitrogen
backbone_size3948
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
cloning vector TT/AA
growth notes :
The murine Myt1 cDNA (6 zinc fingers) had 3 base mutations within the coding region (bp 806, 837 and 4344). These sites were corrected using Stratagene QuickChang Site-Directed mutagenesis. The Myt1 was sequenced after the mutagenesis and found to be correct. There is still one mutation in the 5' UTR at bp 529 (START codon is bp 532)
growth temp :
Normal growth conditions
origin :
37
pi :
alt_names
Myt1
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3T7 promoter
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNzf2, Nzf2a, Nzf2b, Nztf2
geneMyt1
id17932
genbank_ids
mutation
nameMyelin Transcription Factor 1
shRNA_sequence
size5466
species
10090
Mus musculus
tags
plasmid copy :
DNA cloned in Hudson laboratory
resistance markers :
632
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA