This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLH121
catalog :
22696
citations: 1
Reference
Nadon N, Arnheiter H, Hudson L. A combination of PLP and DM20 transgenes promotes partial myelination in the jimpy mouse. J Neurochem. 1994;63:822-33 pubmed
product information
Catalog Number :
22696
Product Name :
pLH121
article :
doi10.1046/j.1471-4159.1994.63030822.x
id2978
pubmed_id7519661
bacterial resistance :
Ampicillin
cloning :
backbonepTZ19R
backbone_mutation
backbone_originFermentas
backbone_size2862
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Phagemid
growth notes :
This was the PLP transgene construct: consists of the human PLP cDNA, flanked on the 5' side by 4.2 kb of PLP promoter sequence from the human genomic DNA and on the 3' side by 1.5 kb of genomic DNA from the 3' end of the human PLP gene.
growth temp :
DH5 alpha (normal growth conditiions)
origin :
37
pi :
alt_names
PLP
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoter
sequencing_primer_3M13/pUC reverse
sequencing_primer_5M13/pUC (-20)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGPM6C, HLD1, MMPL, PLP, PLP/DM20, PMD, SPG2
genePLP1
id5354
genbank_ids
mutation
nameHuman Proteolipid Protein cDNA
shRNA_sequence
size9000
species
9606
Homo sapiens
tags
plasmid copy :
The human PLP genomic and cDNA was cloned in Hudson lab
resistance markers :
632
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA