pBABE-puro-mIR-31 sponge | Addgene 22694 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBABE-puro-mIR-31 sponge

catalog :

22694

product information

Catalog Number :

22694

Product Name :

pBABE-puro-mIR-31 sponge

article :

doi	10.1016/j.cell.2009.03.047
id	2977
pubmed_id	19524507

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE-puro

backbone_mutation

backbone_origin

Weinberg lab (Addgene#:1764)

backbone_size

5169

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

Useful for stable suppression of miR-31 function. Original sponge backbone modifed from Ebert et al., Nat. Methods (2007); miR-31 construct and stable design are novel Repeated sequence is: AGGCAAGACGAGGCATAGCT *Addgene sequencing found at least partial EGFP upstream of the sponge. We do not know if the EGFP is functional or a side-product of the cloning.

growth strain :

Stable suppression of miR-31 function

origin :

pi :

alt_names

stable miR-31 sponge

cloning

clone_method	Restriction Enzyme
cloning_site_3	ApaI
cloning_site_5	BamHI
promoter
sequencing_primer_3	pBabe-3
sequencing_primer_5	pBabe-5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIRN31, hsa-mir-31, miR-31
gene	MIR31
id	407035

genbank_ids

mutation

miR-31 sponge with 7x tandem binding sites to the microRNA.

name

miR-31

shRNA_sequence

size

1100

species

9606

Homo sapiens