pMycMyt1-7zf/IRES-Red | Addgene 22652 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMycMyt1-7zf/IRES-Red

catalog :

22652

citations: 3

Reference
Melhuish T, Kowalczyk I, Manukyan A, Zhang Y, Shah A, Abounader R, et al. Myt1 and Myt1l transcription factors limit proliferation in GBM cells by repressing YAP1 expression. Biochim Biophys Acta Gene Regul Mech. 2018;1861:983-995 pubmed publisher
Manukyan A, Kowalczyk I, Melhuish T, Lemiesz A, Wotton D. Analysis of transcriptional activity by the Myt1 and Myt1l transcription factors. J Cell Biochem. 2018;119:4644-4655 pubmed publisher
Nielsen J, Berndt J, Hudson L, Armstrong R. Myelin transcription factor 1 (Myt1) modulates the proliferation and differentiation of oligodendrocyte lineage cells. Mol Cell Neurosci. 2004;25:111-23 pubmed

product information

Catalog Number :

22652

Product Name :

pMycMyt1-7zf/IRES-Red

article :

doi	10.1016/j.mcn.2003.10.001
id	2967
pubmed_id	14962745

bacterial resistance :

Kanamycin

cloning :

backbone

pIRESRed

backbone_mutation

backbone_origin

R. Armstrong

backbone_size

5635

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Mu Myt1 complete sequence includes 7 Zn Fingers and a Myc Tag. This was subcloned into pIRES/Red made by R. A. Armstrong. The DsRed does not express due to problem with IRES This Myt1-7zf DNA was cut from the full-length clone (pMycMyt1-7zf) which was a gift of Dr. Jacob Hecksher-Sorensen.

growth temp :

DH5 alpha

origin :

pi :

alt_names

Myt1

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3
sequencing_primer_5	N/A
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Nzf2, Nzf2a, Nzf2b, Nztf2
gene	Myt1
id	17932

genbank_ids

mutation

name

Myelin Transcription Factor 1

shRNA_sequence

size

3870

species

10090

Mus musculus