This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJB046
catalog :
22627
citations: 1
Reference
Berndt J, Kim J, Hudson L. Identification of cis-regulatory elements in the myelin proteolipid protein (PLP) gene. J Biol Chem. 1992;267:14730-7 pubmed
product information
Catalog Number :
22627
Product Name :
pJB046
article :
doi
id2961
pubmed_id1378839
bacterial resistance :
Ampicillin
cloning :
backbonep18CAT
backbone_mutation
backbone_originR. McKinnon
backbone_size4728
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
CAT reporter plasmid
growth notes :
Insert is 1200bp (approx) of the Human PLP 5' upstream, promoter region and Exon1 (-1088 to +85) in the correct orientation. bp -13 to -3 (NF-1 like site) were mutated via Site-Directed Mutagenesis from GGCTGTCAATC to cGCTaTgAATg This PLP DNA cloned by Hudson lab.
growth temp :
DH5 alpha
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5HindIII
promoter
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGPM6C, HLD1, MMPL, PLP, PLP/DM20, PMD, SPG2
genePLP1
id5354
genbank_ids
NG_008863
mutationSite 1 (NF-like) GGCTGTCAATC to cGCTaTgAATg
namehuman genomic PLP promoter region
shRNA_sequence
size1088
species
9606
Homo sapiens
tags
resistance markers :
632
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA