pHEF-VSVG | Addgene 22501 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHEF-VSVG

catalog :

22501

citations: 9

Reference
Proulx J, Stacy S, Park I, Borgmann K. A Non-Canonical Role for IRE1α Links ER and Mitochondria as Key Regulators of Astrocyte Dysfunction: Implications in Methamphetamine use and HIV-Associated Neurocognitive Disorders. Front Neurosci. 2022;16:906651 pubmed publisher
Atindaana E, Kissi Twum A, Emery S, Burnett C, Pitcher J, Visser M, et al. Bimodal Expression Patterns, and Not Viral Burst Sizes, Predict the Effects of Vpr on HIV-1 Proviral Populations in Jurkat Cells. MBio. 2022;13:e0374821 pubmed publisher
Benjamin R, Banerjee A, Wu X, Geurink C, Buczek L, Eames D, et al. XRCC4 and MRE11 Roles and Transcriptional Response to Repair of TALEN-Induced Double-Strand DNA Breaks. Int J Mol Sci. 2022;23: pubmed publisher
Nathan A, Rossin E, Kaseke C, Park R, Khatri A, Koundakjian D, et al. Structure-guided T cell vaccine design for SARS-CoV-2 variants and sarbecoviruses. Cell. 2021;: pubmed publisher
Kaseke C, Park R, Singh N, Koundakjian D, Bashirova A, Garcia Beltran W, et al. HLA class-I-peptide stability mediates CD8+ T cell immunodominance hierarchies and facilitates HLA-associated immune control of HIV. Cell Rep. 2021;36:109378 pubmed publisher
Sussman R, Manning B, Ackerman D, Bigdeli A, Pammer P, Velu P, et al. Interpretative differences of combined cytogenetic and molecular profiling highlights differences between MRC and ELN classifications of AML. Cancer Genet. 2021;256-257:68-76 pubmed publisher
Colombo F, Casella G, Podini P, Finardi A, Racchetti G, Norton E, et al. Polarized cells display asymmetric release of extracellular vesicles. Traffic. 2020;: pubmed publisher
Semple Rowland S, Berry J. Use of lentiviral vectors to deliver and express bicistronic transgenes in developing chicken embryos. Methods. 2014;66:466-73 pubmed publisher
Coleman J, Huentelman M, Kasparov S, Metcalfe B, Paton J, Katovich M, et al. Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Physiol Genomics. 2003;12:221-8 pubmed

product information

Catalog Number :

22501

Product Name :

pHEF-VSVG

article :

doi	10.1152/physiolgenomics.00135.2002
id	2951
pubmed_id	12488511

bacterial resistance :

Ampicillin

cloning :

backbone

N/A

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This plasmid forms part of a plasmid set for lentivirus generation in the Kasparov lab, University of Bristol, UK. Addgene was unable to obtain quality control sequence for this plasmid. The depositing lab suggested the following diagnostic digest: PstI digest, expect 0.55, 1.3, and 4.0 kb fragments. Our results for the digest can be seen under Addgene Notes on this page.

growth temp :

Transform into DH5alpha and grow in Luria Broth containing 100 g/ml ampicillin.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	N/A
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

Vesicular Stomatitis Virus Glycoprotein

shRNA_sequence

size

species

Rhabdoviridae