This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAc5.1B-EGFP-DmGW182
catalog :
22419
citations: 1
Reference
Behm Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E. mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes. Genes Dev. 2006;20:1885-98 pubmed
product information
Catalog Number :
22419
Product Name :
pAc5.1B-EGFP-DmGW182
article :
doi10.1101/gad.1424106
id2643
pubmed_id16815998
bacterial resistance :
Ampicillin
cloning :
backbonepAc5.1B-EGFP
backbone_mutation
backbone_originElisa Izaurralde
backbone_size6084
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Drosophila expression
growth temp :
Dh5 alpha and XL1-blue
origin :
37
pi :
alt_names
GW182
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5NotI
promoter
sequencing_primer_3BGH reverse priming site
sequencing_primer_5Ac5 forward primer site
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG31992, CG11484, CG31992, CG9905, Dmel CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182
genegw
id43808
genbank_ids
NM_166780
mutation
namegawky, variant A
shRNA_sequence
size4155
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagEGFP
resistance markers :
223
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA