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product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE-puro mCherry-EGFP-LC3B
catalog :
22418
citations: 167
Reference
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Jutten B, Keulers T, Peeters H, Schaaf M, Savelkouls K, Compter I, et al. EGFRvIII expression triggers a metabolic dependency and therapeutic vulnerability sensitive to autophagy inhibition. Autophagy. 2018;14:283-295 pubmed publisher
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Sun T, Ming L, Yan Y, Zhang Y, Xue H. Beclin 1 acetylation impairs the anticancer effect of aspirin in colorectal cancer cells. Oncotarget. 2017;8:74781-74790 pubmed publisher
Deng Z, Manz D, TORTI S, Torti F. Effects of ferroportin-mediated iron depletion in cells representative of different histological subtypes of prostate cancer. Antioxid Redox Signal. 2017;: pubmed publisher
Dai B, Zhu F, Chen Y, Zhou R, Wang Z, Xie Y, et al. ASIC1a Promotes Acid-Induced Autophagy in Rat Articular Chondrocytes through the AMPK/FoxO3a Pathway. Int J Mol Sci. 2017;18: pubmed publisher
Baksi S, Singh N. α-Synuclein impairs ferritinophagy in the retinal pigment epithelium: Implications for retinal iron dyshomeostasis in Parkinson's disease. Sci Rep. 2017;7:12843 pubmed publisher
Linares J, Cordes T, Duran A, Reina Campos M, Valencia T, Ahn C, et al. ATF4-Induced Metabolic Reprograming Is a Synthetic Vulnerability of the p62-Deficient Tumor Stroma. Cell Metab. 2017;26:817-829.e6 pubmed publisher
Jiao L, Zhang H, Li D, Yang K, Tang J, Li X, et al. Regulation of glycolytic metabolism by autophagy in liver cancer involves selective autophagic degradation of HK2 (hexokinase 2). Autophagy. 2018;14:671-684 pubmed publisher
Sulkshane P, Teni T. BH3 mimetic Obatoclax (GX15-070) mediates mitochondrial stress predominantly via MCL-1 inhibition and induces autophagy-dependent necroptosis in human oral cancer cells. Oncotarget. 2017;8:60060-60079 pubmed publisher
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Lee A, Rayner S, Gwee S, De Luca A, Shahheydari H, Sundaramoorthy V, et al. Pathogenic mutation in the ALS/FTD gene, CCNF, causes elevated Lys48-linked ubiquitylation and defective autophagy. Cell Mol Life Sci. 2018;75:335-354 pubmed publisher
Choi J, Kim H, Bae Y, Cheong H. REP1 Modulates Autophagy and Macropinocytosis to Enhance Cancer Cell Survival. Int J Mol Sci. 2017;18: pubmed publisher
Kim S, Yang C, Lee H, Kim J, Kim Y, Kim Y, et al. ESRRA (estrogen-related receptor α) is a key coordinator of transcriptional and post-translational activation of autophagy to promote innate host defense. Autophagy. 2018;14:152-168 pubmed publisher
Bingel C, Koeneke E, Ridinger J, Bittmann A, Sill M, Peterziel H, et al. Three-dimensional tumor cell growth stimulates autophagic flux and recapitulates chemotherapy resistance. Cell Death Dis. 2017;8:e3013 pubmed publisher
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Kim S, Kwon H, Im S, Son Y, Akindehin S, Jung Y, et al. Connexin 43 is required for the maintenance of mitochondrial integrity in brown adipose tissue. Sci Rep. 2017;7:7159 pubmed publisher
Wang N, Tan H, Li S, Feng Y. Atg9b Deficiency Suppresses Autophagy and Potentiates Endoplasmic Reticulum Stress-Associated Hepatocyte Apoptosis in Hepatocarcinogenesis. Theranostics. 2017;7:2325-2338 pubmed publisher
Kuzu O, Gowda R, Noory M, Robertson G. Modulating cancer cell survival by targeting intracellular cholesterol transport. Br J Cancer. 2017;117:513-524 pubmed publisher
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Kaufman D, Papillon J, Larose L, Iwawaki T, Cybulsky A. Deletion of inositol-requiring enzyme-1? in podocytes disrupts glomerular capillary integrity and autophagy. Mol Biol Cell. 2017;28:1636-1651 pubmed publisher
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Zummo F, Cullen K, Honkanen Scott M, Shaw J, Lovat P, Arden C. Glucagon-Like Peptide 1 Protects Pancreatic ?-Cells From Death by Increasing Autophagic Flux and Restoring Lysosomal Function. Diabetes. 2017;66:1272-1285 pubmed publisher
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product information
Catalog Number :
22418
Product Name :
pBABE-puro mCherry-EGFP-LC3B
article :
doi10.1038/embor.2008.238
id6674
pubmed_id19148225
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Please click the Notes from Addgene link above for sequence submitted by a requesting scientist and further observations on using this plasmid.
growth strain :
retroviral expression of mCherry, EGFP-tagged LC3
origin :
37
pi :
alt_names
MAP1LC3B
LC3B
LC3
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
geneMAP1LC3B
id81631
genbank_ids
BC045759
mutation
namemicrotubule-associated protein 1 light chain 3 beta
shRNA_sequence
size1900
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmCherry
locationN terminal on insert
tageGFP
plasmid copy :
pDest-mCherry-EGFP-hLC3b from Terje Johansen from the Biochemistry Department, Institute of Medical Biology, University of Troms l.
resistance markers :
636
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA