This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABEpuro GFP-LC3
catalog :
22405
citations: 79
Reference
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Sun T, Ming L, Yan Y, Zhang Y, Xue H. Beclin 1 acetylation impairs the anticancer effect of aspirin in colorectal cancer cells. Oncotarget. 2017;8:74781-74790 pubmed publisher
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Fliss P, Jowers T, Brinkmann M, Holstermann B, Mack C, Dickinson P, et al. Viral mediated redirection of NEMO/IKK? to autophagosomes curtails the inflammatory cascade. PLoS Pathog. 2012;8:e1002517 pubmed publisher
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product information
Catalog Number :
22405
Product Name :
pBABEpuro GFP-LC3
article :
doi10.1091/mbc.e07-10-1092
id2930
pubmed_id18094039
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutationGFP-LC3 was subcloned from pEGFPC1-LC3 into the SnaB1 and Sal1 sites of pBABEpuro
backbone_originAddgene plasmid # 1764
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. Kabeya Y et al. (EMBO J. 2000 Nov 1. 19(21):5720-8.
growth strain :
retroviral expression of GFP-tagged LC3
origin :
37
pi :
alt_names
Map1lc3b
LC3b
LC3
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5SnaBI
promoter
sequencing_primer_3pBABE-3
sequencing_primer_5pBABE-5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLC3B, Map1lc3, Mpl3, zbs559
geneMap1lc3b
id64862
genbank_ids
U05784
mutation
namemicrotubule-associated protein 1 light chain 3 beta
shRNA_sequence
size1500
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagGFP
plasmid copy :
pEGFPC1-LC3 from Tamotsu Yoshimori at the National Institute of Genetics, Mishima, Japan.
resistance markers :
636
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA