This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pR5-19
catalog :
22288
citations: 2
Reference
Elserafy M, El Shiekh I, Fleifel D, Atteya R, AlOkda A, Abdrabbou M, et al. A role for Rad5 in ribonucleoside monophosphate (rNMP) tolerance. Life Sci Alliance. 2021;4: pubmed publisher
Pages V, Bresson A, Acharya N, Prakash S, Fuchs R, Prakash L. Requirement of Rad5 for DNA polymerase zeta-dependent translesion synthesis in Saccharomyces cerevisiae. Genetics. 2008;180:73-82 pubmed publisher
product information
Catalog Number :
22288
Product Name :
pR5-19
article :
doi10.1534/genetics.108.091066
id2908
pubmed_id18757916
bacterial resistance :
Ampicillin
cloning :
backboneYCplac111
backbone_mutation
backbone_origin
backbone_size6112
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Mutation abolishes the ubiquitin ligase function
origin :
37
pi :
alt_names
rad5-Ub ligase mutant
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13_puc-for
sequencing_primer_5M13_pUC-rev
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYLR032W, REV2, SNM2
geneRAD5
id850719
genbank_ids
mutationC914, C917->AA in the C3HC4 ring-finger motif
nameRAD5
shRNA_sequence
size3510
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
627
tags :
Unknown
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA