pVP64 | Addgene 22287 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pVP64

catalog :

22287

citations: 1

Reference
Pages V, Santa Maria S, Prakash L, Prakash S. Role of DNA damage-induced replication checkpoint in promoting lesion bypass by translesion synthesis in yeast. Genes Dev. 2009;23:1438-49 pubmed publisher

product information

Catalog Number :

22287

Product Name :

pVP64

article :

doi	10.1101/gad.1793409
id	2907
pubmed_id	19528320

bacterial resistance :

Ampicillin

cloning :

backbone

YCplac111

backbone_mutation

backbone_origin

backbone_size

6112

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

The REV1 ORF plus 1050 nucleotides (nt) of its upstream region that include the REV1 promoter and 500nt of the downstream region that include the REV1 transcription terminator was cloned in the vector YCplac111, which carries the LEU2 gene, the autonomous replicating sequence ARS1, and the centromeric CEN4 region. A BglII restriction site was added by site-directed mutagenesis right before the stop codon of REV1. A 33-mer oligonucleotide duplex containing the HA-coding sequence and a BamHI and a BglII restriction site was inserted into the BglII restriction site, adding an HA tag in fusion with REV1, and regenerating a unique BglII restriction site. This step was repeated three times in order to get four HA tags in fusion with REV1.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII (between insert and tag)
cloning_site_5
promoter
sequencing_primer_3	M13_puc-for
sequencing_primer_5	M13_pUC-rev
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YOR346W
gene	REV1
id	854527

genbank_ids

mutation

name

REV1

shRNA_sequence

size

4640

species

4932

Saccharomyces cerevisiae