This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAL204
catalog :
22282
citations: 2
Reference
Adrian M, Nijenhuis W, Hoogstraaten R, Willems J, Kapitein L. A Phytochrome-Derived Photoswitch for Intracellular Transport. ACS Synth Biol. 2017;6:1248-1256 pubmed publisher
Levskaya A, Weiner O, Lim W, Voigt C. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009;461:997-1001 pubmed publisher
product information
Catalog Number :
22282
Product Name :
pAL204
article :
doi10.1038/nature08446
id2903
pubmed_id19749742
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Synthetic Biology
growth notes :
Kras4B [Entrez Gene ID: 3845] polybasic CaaX terminus (KKKKKKSKTKCVIM) [ggt aaa aag aag aaa aag aag tca aag aca aag tgt gta att atg].
growth strain :
Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
origin :
37
pi :
alt_names
phyB
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1
cloning_site_5Kpn1
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
genePHYB
id816394
genbank_ids
mutation
namepCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT
shRNA_sequence
size3551
species
3702
Arabidopsis thaliana
tags
resistance markers :
626
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA