This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAL149
catalog :
22275
citations: 2
Reference
Yüz S, Ricken J, Wegner S. Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions. Adv Sci (Weinh). 2018;5:1800446 pubmed publisher
Levskaya A, Weiner O, Lim W, Voigt C. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009;461:997-1001 pubmed publisher
product information
Catalog Number :
22275
Product Name :
pAL149
article :
doi10.1038/nature08446
id2903
pubmed_id19749742
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Synthetic Biology
growth strain :
Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
origin :
37
pi :
alt_names
phyB
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1
cloning_site_5Kpn1
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
genePHYB
id816394
genbank_ids
mutation
namePhyB(1-908)
shRNA_sequence
size3542
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagmCherry-Kras4BCT (prenylation sequence)
resistance markers :
626
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA