This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti CMV/TO RasV12 Puro (w119-1)
catalog :
22262
citations: 10
Reference
Toropov A, Deryabin P, Shatrova A, Borodkina A. Oncogene-Induced Senescence Is a Crucial Antitumor Defense Mechanism of Human Endometrial Stromal Cells. Int J Mol Sci. 2023;24: pubmed publisher
Raymonda M, Rodr xed guez S xe1 nchez I, Schafer X, Smorodintsev Schiller L, Harris I, Munger J. Cytomegalovirus-induced inactivation of TSC2 disrupts the coupling of fatty acid biosynthesis to glucose availability resulting in a vulnerability to glucose limitation. bioRxiv. 2023;: pubmed publisher
Raymonda M, Ciesla J, Monaghan M, Leach J, Asantewaa G, Smorodintsev Schiller L, et al. Pharmacologic profiling reveals lapatinib as a novel antiviral against SARS-CoV-2 in vitro. Virology. 2022;566:60-68 pubmed publisher
Ryu S, Kim B, Cho H, Park C, Choi H, Jang E, et al. Disruption of nucleocytoplasmic trafficking as a cellular senescence driver. Exp Mol Med. 2021;53:1092-1108 pubmed publisher
He Y, Zhang X, Chang J, Kim H, Zhang P, Wang Y, et al. Using proteolysis-targeting chimera technology to reduce navitoclax platelet toxicity and improve its senolytic activity. Nat Commun. 2020;11:1996 pubmed publisher
Santos L, Colman L, Contreras P, Chini C, Carlomagno A, Leyva A, et al. A novel form of Deleted in breast cancer 1 (DBC1) lacking the N-terminal domain does not bind SIRT1 and is dynamically regulated in vivo. Sci Rep. 2019;9:14381 pubmed publisher
Yoh K, Regunath K, Guzman A, Lee S, Pfister N, Akanni O, et al. Repression of p63 and induction of EMT by mutant Ras in mammary epithelial cells. Proc Natl Acad Sci U S A. 2016;113:E6107-E6116 pubmed
Xu S, Schafer X, Munger J. Expression of Oncogenic Alleles Induces Multiple Blocks to Human Cytomegalovirus Infection. J Virol. 2016;90:4346-4356 pubmed publisher
Trucco L, Andreoli V, Núñez N, Maccioni M, Bocco J. Krüppel-like factor 6 interferes with cellular transformation induced by the H-ras oncogene. FASEB J. 2014;28:5262-76 pubmed publisher
Zolochevska O, Figueiredo M. Cell-cycle regulators cdk2ap1 and bicalutamide suppress malignant biological interactions between prostate cancer and bone cells. Prostate. 2011;71:353-67 pubmed publisher
product information
Catalog Number :
22262
Product Name :
pLenti CMV/TO RasV12 Puro (w119-1)
article :
doi
id2881
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
backbone_mutation
backbone_origin
backbone_size8176
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth temp :
Stbl3, 37oC
origin :
37
pi :
alt_names
RasV12
cloning
clone_methodRestriction Enzyme
cloning_site_3Xba I
cloning_site_5Sal I
promoter
sequencing_primer_3n/a
sequencing_primer_5CMVforw
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutationG12V mutant
nameHRAS G12V
shRNA_sequence
size569
species
9606
Homo sapiens
tags
plasmid copy :
RasV12 cDNA was obtained from Dr. Christian Beausejour.
resistance markers :
63
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA