This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti CMV RasV12 Neo (w108-1)
catalog :
22259
citations: 4
Reference
Kumar B, Khatpe A, Guanglong J, Batic K, Bhat Nakshatri P, Granatir M, et al. Stromal heterogeneity may explain increased incidence of metaplastic breast cancer in women of African descent. Nat Commun. 2023;14:5683 pubmed publisher
Kumar B, Bhat Nakshatri P, Maguire C, Jacobsen M, Temm C, Sandusky G, et al. Bidirectional Regulatory Cross-Talk between Cell Context and Genomic Aberrations Shapes Breast Tumorigenesis. Mol Cancer Res. 2021;: pubmed publisher
Bryson B, Tamagno I, Taylor S, Parameswaran N, Chernosky N, Balasubramaniam N, et al. Aberrant Induction of a Mesenchymal/Stem Cell Program Engages Senescence in Normal Mammary Epithelial Cells. Mol Cancer Res. 2020;: pubmed publisher
Sancho Martinez I, Nivet E, Xia Y, Hishida T, Aguirre A, Ocampo A, et al. Establishment of human iPSC-based models for the study and targeting of glioma initiating cells. Nat Commun. 2016;7:10743 pubmed publisher
product information
Catalog Number :
22259
Product Name :
pLenti CMV RasV12 Neo (w108-1)
article :
doi
id2881
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
backbone_mutation
backbone_origin
backbone_size8378
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth temp :
Stbl3, 37oC
origin :
37
pi :
alt_names
RasV12
cloning
clone_methodRestriction Enzyme
cloning_site_3Xba I
cloning_site_5Sal I
promoter
sequencing_primer_3n/a
sequencing_primer_5CMVforw
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutationG12V mutant
nameHRAS G12V
shRNA_sequence
size569
species
9606
Homo sapiens
tags
plasmid copy :
RasV12 cDNA obtained from Dr. Christian Beausejour.
resistance markers :
63
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA