pEGFPC1-EPSIN 1 | Addgene 22228 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFPC1-EPSIN 1

catalog :

22228

citations: 7

Reference
Ogunmowo T, Jing H, Raychaudhuri S, Kusick G, Imoto Y, Li S, et al. Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis. Nat Commun. 2023;14:2888 pubmed publisher
Yang C, Colosi P, Hugelier S, Zabezhinsky D, Lakadamyali M, Svitkina T. Actin polymerization promotes invagination of flat clathrin-coated lattices in mammalian cells by pushing at lattice edges. Nat Commun. 2022;13:6127 pubmed publisher
G xfc nther S, Mart xed nez Romero C, Sempere Borau M, Pham C, Garc xed a Sastre A, Stertz S. Proteomic Identification of Potential Target Proteins of Cathepsin W for Its Development as a Drug Target for Influenza. Microbiol Spectr. 2022;10:e0092122 pubmed publisher
Li J, Barylko B, Eichorst J, MUELLER J, Albanesi J, Chen Y. Association of Endophilin B1 with Cytoplasmic Vesicles. Biophys J. 2016;111:565-576 pubmed publisher
Okano M, Matsuo H, Nishimura Y, Hozumi K, Yoshioka S, Tonoki A, et al. Mib1 modulates dynamin 2 recruitment via Snx18 to promote Dll1 endocytosis for efficient Notch signaling. Genes Cells. 2016;21:425-41 pubmed publisher
Sochacki K, Shtengel G, Van Engelenburg S, Hess H, Taraska J. Correlative super-resolution fluorescence and metal-replica transmission electron microscopy. Nat Methods. 2014;11:305-8 pubmed publisher
Chen H, Fre S, Slepnev V, Capua M, Takei K, Butler M, et al. Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis. Nature. 1998;394:793-7 pubmed

product information

Catalog Number :

22228

Product Name :

pEGFPC1-EPSIN 1

article :

doi	10.1038/29555
id	2894
pubmed_id	9723620

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Bacterial Expression

growth notes :

Please note: Addgene's quality control sequence found the EPSIN1 gene has an insertion of threonine after aa 821 and since there is no stop codon, ESPIN1 contains the additional c terminal residues VDGTAGPGSTGSR. The depositor noted that these discrepancies do NOT affect plasmid function.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	EGFP-C Sequencing Primer
sequencing_primer_5	EGFP-N Sequencing Primere
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Epn1
id	117277

genbank_ids

AF018261

mutation

please see depositor comment below

name

Epsin 1

shRNA_sequence

size

1800

species

10116

Rattus norvegicus