This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSFG-TFII-I-GFP Y248&249F
catalog :
22196
citations: 1
Reference
Desgranges Z, Ahn J, Lazebnik M, Ashworth T, Lee C, Pestell R, et al. Inhibition of TFII-I-dependent cell cycle regulation by p53. Mol Cell Biol. 2005;25:10940-52 pubmed
product information
Catalog Number :
22196
Product Name :
pSFG-TFII-I-GFP Y248&249F
article :
doi10.1128/MCB.25.24.10940-10952.2005
id2880
pubmed_id16314517
bacterial resistance :
Ampicillin
cloning :
backbonepSFG
backbone_mutation
backbone_origin
backbone_size8900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
This vector was derived from pSFG-tcLuc (Lindemann et al., 1998). Important: There may be a point mutation, Tyrosine 611 changed to Phenylalanine
origin :
37
pi :
alt_names
TF-II-I delta isoform
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NcoI
promoter
sequencing_primer_3
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6
geneGTF2I
id2969
genbank_ids
mutationChanged Tyrosines 248 and 249 to Phenylalanines. Important: There may be a point mutation, Tyrosine 611 changed to Phenylalanine
nameTFII-I
shRNA_sequence
size2900
species
9606
Homo sapiens
tags
locationC terminal on insert
tagGFP
resistance markers :
621
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA