This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HA-HIF2 P405A;P531A;bHLH*-pBabe-Puro
catalog :
22135
citations: 1
Reference
Kondo K, Kim W, Lechpammer M, Kaelin W. Inhibition of HIF2alpha is sufficient to suppress pVHL-defective tumor growth. PLoS Biol. 2003;1:E83 pubmed
product information
Catalog Number :
22135
Product Name :
HA-HIF2 P405A;P531A;bHLH*-pBabe-Puro
article :
doi10.1371/journal.pbio.0000083
id2856
pubmed_id14691554
bacterial resistance :
Ampicillin
cloning :
backbonepBabe-Puro
backbone_mutation
backbone_origin
backbone_size5188
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth temp :
DH5alpha BL21
origin :
37
pi :
alt_names
HIF2a
EPAS1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR I
cloning_site_5BamH I
promoter
sequencing_primer_3pBabe-3'
sequencing_primer_5pBabe-5'
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73
geneEPAS1
id2034
genbank_ids
NM_001430
mutationcDNA changed amino acids from wt: Proline 405 to Alanine Proline 531 to Alanine mutation in bHLH region changed amino acids 24-29 from wt: RCRRSK to ACAASA
nameHypoxia inducible factor 2 alpha
shRNA_sequence
size2655
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA-tag
resistance markers :
238
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA