This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AAVS1 hPGK-PuroR-pA donor
catalog :
22072
citations: 15
Reference
Roudaut M, Idriss S, Caillaud A, Girardeau A, Rimbert A, Champon B, et al. PCSK9 regulates the NODAL signaling pathway and cellular proliferation in hiPSCs. Stem Cell Reports. 2021;16:2958-2972 pubmed publisher
Han J, LaVigne C, Jones B, Zhang H, Gillett F, Mendell J. A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming. Science. 2020;370: pubmed publisher
Molenaar T, Pagès Gallego M, Meyn V, van Leeuwen F. Application of Recombination -Induced Tag Exchange (RITE) to study histone dynamics in human cells. Epigenetics. 2020;:1-13 pubmed publisher
Manjunath H, Zhang H, Rehfeld F, Han J, Chang T, Mendell J. Suppression of Ribosomal Pausing by eIF5A Is Necessary to Maintain the Fidelity of Start Codon Selection. Cell Rep. 2019;29:3134-3146.e6 pubmed publisher
Elguindy M, Kopp F, Goodarzi M, Rehfeld F, Thomas A, Chang T, et al. PUMILIO, but not RBMX, binding is required for regulation of genomic stability by noncoding RNA NORAD. elife. 2019;8: pubmed publisher
Li Y, Kong Q, Yue J, Gou X, Xu M, Wu X. Genome-edited skin epidermal stem cells protect mice from cocaine-seeking behaviour and cocaine overdose. Nat Biomed Eng. 2019;3:105-113 pubmed publisher
Du J, Kirk B, Zeng J, Ma J, Wang Q. Three classes of response elements for human PRC2 and MLL1/2-Trithorax complexes. Nucleic Acids Res. 2018;46:8848-8864 pubmed publisher
Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, et al. Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation. ACS Chem Biol. 2018;13:609-617 pubmed publisher
Yue J, Gou X, Li Y, Wicksteed B, Wu X. Engineered Epidermal Progenitor Cells Can Correct Diet-Induced Obesity and Diabetes. Cell Stem Cell. 2017;21:256-263.e4 pubmed publisher
Hafner M, Niepel M, Chung M, Sorger P. Growth rate inhibition metrics correct for confounders in measuring sensitivity to cancer drugs. Nat Methods. 2016;13:521-7 pubmed publisher
Zhou J, Wang C, Zhang K, Wang Y, Gong X, Wang Y, et al. Generation of Human Embryonic Stem Cell Line Expressing zsGreen in Cholinergic Neurons Using CRISPR/Cas9 System. Neurochem Res. 2016;41:2065-74 pubmed publisher
Natsume T, Kiyomitsu T, Saga Y, Kanemaki M. Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Cell Rep. 2016;15:210-218 pubmed publisher
Lee S, Kopp F, Chang T, Sataluri A, Chen B, Sivakumar S, et al. Noncoding RNA NORAD Regulates Genomic Stability by Sequestering PUMILIO Proteins. Cell. 2016;164:69-80 pubmed publisher
Dreyer A, Cathomen T. Zinc-finger nucleases-based genome engineering to generate isogenic human cell lines. Methods Mol Biol. 2012;813:145-56 pubmed publisher
Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, DeKelver R, et al. Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol. 2009;27:851-7 pubmed publisher
product information
Catalog Number :
22072
Product Name :
AAVS1 hPGK-PuroR-pA donor
article :
doi10.1038/nbt.1562
id2848
pubmed_id19680244
bacterial resistance :
Ampicillin
cloning :
backboneN/A
backbone_mutation
backbone_origin
backbone_size5876
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
ZFN donor
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5N/A
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-S-68p, MIG10, PGKA
genePGK1
id5230
genbank_ids
mutation
namePGK-Puro
shRNA_sequence
size1105
species
9606
Homo sapiens
tags
resistance markers :
227
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA