This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTriEx-mCherry-PA-Rac1-T17N
catalog :
22029
citations: 4
Reference
Fan X, Ma C, Song J, Liao Z, Huang N, Liu X, et al. Rac1 Signaling in Amygdala Astrocytes Regulates Fear Memory Acquisition and Retrieval. Neurosci Bull. 2021;37:947-958 pubmed publisher
Kato T, Kawai K, Egami Y, Kakehi Y, Araki N. Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity. PLoS ONE. 2014;9:e97749 pubmed publisher
Sakurai T, Woolls M, Jin S, Murakami M, Simons M. Inter-cellular exchange of cellular components via VE-cadherin-dependent trans-endocytosis. PLoS ONE. 2014;9:e90736 pubmed publisher
Wu Y, Frey D, Lungu O, Jaehrig A, Schlichting I, Kuhlman B, et al. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009;461:104-8 pubmed publisher
product information
Catalog Number :
22029
Product Name :
pTriEx-mCherry-PA-Rac1-T17N
article :
doi10.1038/nature08241
id2870
pubmed_id19693014
bacterial resistance :
Ampicillin
cloning :
backbonepTriEx
backbone_mutation
backbone_originNovagen
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
Insect Expression
growth strain :
Expression of photoactivatable Rac1 (dominant-negative mutant).
origin :
37
pi :
alt_names
LOV2-Rac1
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5Bam HI
promoterCMV, p10
sequencing_primer_3
sequencing_primer_5pTriExUP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
AAA36544
mutationLOV(Leu404-Leu546). Rac1 starts at Ile4 and contains mutations Q61L, E91H, N92H, and T17N.
namePA-Rac1-T17N
shRNA_sequence
size1100
species
9606
Homo sapiens
32644
Other
Avena sativa (oat)
tags
locationN terminal on backbone
tag6xHis
locationN terminal on backbone
tagmCherry
resistance markers :
185
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA