pTriEx-mCherry-PA-Rac1-C450A | Addgene 22028 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTriEx-mCherry-PA-Rac1-C450A

catalog :

22028

citations: 4

Reference
Fan X, Ma C, Song J, Liao Z, Huang N, Liu X, et al. Rac1 Signaling in Amygdala Astrocytes Regulates Fear Memory Acquisition and Retrieval. Neurosci Bull. 2021;37:947-958 pubmed publisher
Harris J, Wang A, Boulanger Weill J, Santoriello C, Foianini S, Lichtman J, et al. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects. Dev Cell. 2020;53:577-588.e7 pubmed publisher
Liao Z, Tao Y, Guo X, Cheng D, Wang F, Liu X, et al. Fear Conditioning Downregulates Rac1 Activity in the Basolateral Amygdala Astrocytes to Facilitate the Formation of Fear Memory. Front Mol Neurosci. 2017;10:396 pubmed publisher
Wu Y, Frey D, Lungu O, Jaehrig A, Schlichting I, Kuhlman B, et al. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009;461:104-8 pubmed publisher

product information

Catalog Number :

22028

Product Name :

pTriEx-mCherry-PA-Rac1-C450A

article :

doi	10.1038/nature08241
id	2870
pubmed_id	19693014

bacterial resistance :

Ampicillin

cloning :

backbone

pTriEx

backbone_mutation

backbone_origin

Novagen

backbone_size

6000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Bacterial Expression

Insect Expression

growth strain :

Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

origin :

pi :

alt_names

LOV2-Rac1

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	Bam HI
promoter	CMV, p10
sequencing_primer_3
sequencing_primer_5	pTriExUP
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIG5, MRD48, Rac-1, TC-25, p21-Rac1
gene	RAC1
id	5879

genbank_ids

AAA36544

mutation

LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 and contains mutations Q61L, E91H and N92H.

name

PA-Rac1-C450A

shRNA_sequence

size

1100

species

9606

Homo sapiens

32644

Other

Avena sativa (oat)