This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTriEx-mCherry-PA-Rac1
catalog :
22027
citations: 22
Reference
Peng Y, Chen Z, He Y, Li P, Chen Y, Chen X, et al. Non-muscle myosin II isoforms orchestrate substrate stiffness sensing to promote cancer cell contractility and migration. Cancer Lett. 2022;524:245-258 pubmed publisher
Arts J, Mahlandt E, Grönloh M, Schimmel L, Noordstra I, Gordon E, et al. Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration. elife. 2021;10: pubmed publisher
Fan X, Ma C, Song J, Liao Z, Huang N, Liu X, et al. Rac1 Signaling in Amygdala Astrocytes Regulates Fear Memory Acquisition and Retrieval. Neurosci Bull. 2021;37:947-958 pubmed publisher
Leyden F, Uthishtran S, Moorthi U, York H, Patil A, Gandhi H, et al. Rac1 activation can generate untemplated, lamellar membrane ruffles. BMC Biol. 2021;19:72 pubmed publisher
Park E, Kawai K, Egami Y, Araki N. A novel DENND1B-localized structure found at the basal side of adherent cells. Histochem Cell Biol. 2021;155:9-18 pubmed publisher
Whitelaw J, Swaminathan K, Kage F, Machesky L. The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration. Cells. 2020;9: pubmed publisher
Harris J, Wang A, Boulanger Weill J, Santoriello C, Foianini S, Lichtman J, et al. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects. Dev Cell. 2020;53:577-588.e7 pubmed publisher
van Geel O, Cheung S, Gadella T. Combining optogenetics with sensitive FRET imaging to monitor local microtubule manipulations. Sci Rep. 2020;10:6034 pubmed publisher
Hao H, Niu J, Xue B, Su Q, Liu M, Yang J, et al. Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration. EMBO Rep. 2020;21:e48385 pubmed publisher
Laufer J, Hauser M, Kindinger I, Purvanov V, Pauli A, Legler D. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation. Cell Rep. 2019;29:995-1009.e6 pubmed publisher
Yang J, Bhattacharya S, West Foyle H, Hung C, Wu T, Iglesias P, et al. Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nat Commun. 2018;9:4673 pubmed publisher
van Haren J, Charafeddine R, Ettinger A, Wang H, Hahn K, Wittmann T. Local control of intracellular microtubule dynamics by EB1 photodissociation. Nat Cell Biol. 2018;20:252-261 pubmed publisher
Liao Z, Tao Y, Guo X, Cheng D, Wang F, Liu X, et al. Fear Conditioning Downregulates Rac1 Activity in the Basolateral Amygdala Astrocytes to Facilitate the Formation of Fear Memory. Front Mol Neurosci. 2017;10:396 pubmed publisher
Ikeda Y, Kawai K, Ikawa A, Kawamoto K, Egami Y, Araki N. Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation. J Cell Sci. 2017;130:2530-2540 pubmed publisher
Qudrat A, Mosabbir A, Truong K. LOV2-Controlled Photoactivation of Protein Trans-Splicing. Methods Mol Biol. 2017;1495:227-237 pubmed
He L, Zhang Y, Ma G, Tan P, Li Z, Zang S, et al. Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. elife. 2015;4: pubmed publisher
Wong S, Mosabbir A, Truong K. An Engineered Split Intein for Photoactivated Protein Trans-Splicing. PLoS ONE. 2015;10:e0135965 pubmed publisher
Kato T, Kawai K, Egami Y, Kakehi Y, Araki N. Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity. PLoS ONE. 2014;9:e97749 pubmed publisher
Sakurai T, Woolls M, Jin S, Murakami M, Simons M. Inter-cellular exchange of cellular components via VE-cadherin-dependent trans-endocytosis. PLoS ONE. 2014;9:e90736 pubmed publisher
Wu J, Kent I, Shekhar N, Chancellor T, Mendonca A, Dickinson R, et al. Actomyosin pulls to advance the nucleus in a migrating tissue cell. Biophys J. 2014;106:7-15 pubmed publisher
Pham E, Mills E, Truong K. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol. 2011;18:880-90 pubmed publisher
Wu Y, Frey D, Lungu O, Jaehrig A, Schlichting I, Kuhlman B, et al. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009;461:104-8 pubmed publisher
product information
Catalog Number :
22027
Product Name :
pTriEx-mCherry-PA-Rac1
article :
doi10.1038/nature08241
id2870
pubmed_id19693014
bacterial resistance :
Ampicillin
cloning :
backbonepTriEx
backbone_mutation
backbone_originNovagen
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
Insect Expression
growth strain :
Expression of photoactivatable Rac1
origin :
37
pi :
alt_names
LOV2-Rac1
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5Bam HI
promoterCMV, p10
sequencing_primer_3
sequencing_primer_5pTriExUP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
AAA36544
mutationLOV(Leu404-Leu546), Rac1 starts at Ile4 and contains mutations Q61L, E91H and N92H
namePA-Rac1
shRNA_sequence
size1100
species
9606
Homo sapiens
32644
Other
Avena sativa (oat)
tags
locationN terminal on backbone
tag6xHis
locationN terminal on backbone
tagmCherry
resistance markers :
185
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA