This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTriEx-PA-Rac1-C450A
catalog :
22025
citations: 2
Reference
Hülsemann M, Verkhusha P, Guo P, Miskolci V, Cox D, Hodgson L. Optogenetics: Rho GTPases Activated by Light in Living Macrophages. Methods Mol Biol. 2020;2108:281-293 pubmed publisher
Wu Y, Frey D, Lungu O, Jaehrig A, Schlichting I, Kuhlman B, et al. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009;461:104-8 pubmed publisher
product information
Catalog Number :
22025
Product Name :
pTriEx-PA-Rac1-C450A
article :
doi10.1038/nature08241
id2870
pubmed_id19693014
bacterial resistance :
Ampicillin
cloning :
backbonepTriEx
backbone_mutation
backbone_originNovagen
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
Insect Expression
growth strain :
Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
origin :
37
pi :
alt_names
LOV(Leu404-Leu546 , C450A)-Rac1(Ile4)-Q61L/E91H/N92H
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5Bam HI
promoterCMV, p10
sequencing_primer_3
sequencing_primer_5pTriExUP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
mutationLOV domain contains light-insensitive mutation C450A.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H.
namePA-Rac1-C450A
shRNA_sequence
size1100
species
9606
Homo sapiens
32644
Other
Avena sativa (oat)
tags
locationN terminal on backbone
tag6X His
resistance markers :
185
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA