pTriEx-PA-Rac1 | Addgene 22024 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTriEx-PA-Rac1

catalog :

22024

citations: 4

Reference
Burute M, Jansen K, Mihajlovic M, Vermonden T, Kapitein L. Local changes in microtubule network mobility instruct neuronal polarization and axon specification. Sci Adv. 2022;8:eabo2343 pubmed publisher
Ma G, He L, Liu S, Xie J, Huang Z, Jing J, et al. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun. 2020;11:1039 pubmed publisher
Hongdusit A, Zwart P, Sankaran B, Fox J. Minimally disruptive optical control of protein tyrosine phosphatase 1B. Nat Commun. 2020;11:788 pubmed publisher
Wu Y, Frey D, Lungu O, Jaehrig A, Schlichting I, Kuhlman B, et al. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009;461:104-8 pubmed publisher

product information

Catalog Number :

22024

Product Name :

pTriEx-PA-Rac1

article :

doi	10.1038/nature08241
id	2870
pubmed_id	19693014

bacterial resistance :

Ampicillin

cloning :

backbone

pTriEx

backbone_mutation

backbone_origin

Novagen

backbone_size

6000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Bacterial Expression

Insect Expression

growth strain :

Expression of photoactivatable Rac1

origin :

pi :

alt_names

LOV(Leu404-Leu546)-Rac1(Ile4)-Q61L/E91H/N92H

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	Bam HI
promoter	CMV, p10
sequencing_primer_3
sequencing_primer_5	pTriExUP
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIG5, MRD48, Rac-1, TC-25, p21-Rac1
gene	RAC1
id	5879

genbank_ids

mutation

Rac1 starts at I4 and contains mutations Q61L, E91H and N92H.

name

PA-Rac1

shRNA_sequence

size

1100

species

9606

Homo sapiens

32644

Other

Avena sativa (oat)