This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTriEx-mVenus-PA-Rac1
catalog :
22007
citations: 8
Reference
Leyden F, Uthishtran S, Moorthi U, York H, Patil A, Gandhi H, et al. Rac1 activation can generate untemplated, lamellar membrane ruffles. BMC Biol. 2021;19:72 pubmed publisher
Hülsemann M, Verkhusha P, Guo P, Miskolci V, Cox D, Hodgson L. Optogenetics: Rho GTPases Activated by Light in Living Macrophages. Methods Mol Biol. 2020;2108:281-293 pubmed publisher
Nguyen T, Park W, Park B, Kim C, Oh Y, Kim J, et al. PLEKHG3 enhances polarized cell migration by activating actin filaments at the cell front. Proc Natl Acad Sci U S A. 2016;113:10091-6 pubmed publisher
Kim J, Lee M, Kim N, Heo W. Optogenetic toolkit reveals the role of Ca2+ sparklets in coordinated cell migration. Proc Natl Acad Sci U S A. 2016;113:5952-7 pubmed publisher
Cazaux S, Sadoun A, Biarnes Pelicot M, Martinez M, Obeid S, Bongrand P, et al. Synchronizing atomic force microscopy force mode and fluorescence microscopy in real time for immune cell stimulation and activation studies. Ultramicroscopy. 2016;160:168-181 pubmed publisher
Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, et al. Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Nat Commun. 2015;6:8021 pubmed publisher
Kakumoto T, Nakata T. Optogenetic control of PIP3: PIP3 is sufficient to induce the actin-based active part of growth cones and is regulated via endocytosis. PLoS ONE. 2013;8:e70861 pubmed publisher
Wu Y, Frey D, Lungu O, Jaehrig A, Schlichting I, Kuhlman B, et al. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009;461:104-8 pubmed publisher
product information
Catalog Number :
22007
Product Name :
pTriEx-mVenus-PA-Rac1
article :
doi10.1038/nature08241
id2870
pubmed_id19693014
bacterial resistance :
Ampicillin
cloning :
backbonepTriEx
backbone_mutation
backbone_originNovagen
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
Insect Expression
growth strain :
Expression of photoactivatable Rac1
origin :
37
pi :
alt_names
LOV2-Rac1
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5Bam HI
promoterCMV, p10
sequencing_primer_3
sequencing_primer_5pTriExUP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
mutationLOV(Leu404-Leu546), Rac1 starts at I4 and contains mutations Q61L, E91H and N92H.
namePA-Rac1
shRNA_sequence
size1100
species
9606
Homo sapiens
32644
Other
Avena sativa (oat)
tags
locationN terminal on backbone
tag6X His
locationN terminal on backbone
tagmVenus
resistance markers :
185
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA