BiP 2: C. elegans BiP (hsp-4)-GFP | Addgene 21896 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

BiP 2: C. elegans BiP (hsp-4)-GFP

catalog :

21896

citations: 2

Reference
Zha J, Ying M, Alexander Floyd J, Gidalevitz T. HSP-4/BiP expression in secretory cells is regulated by a developmental program and not by the unfolded protein response. PLoS Biol. 2019;17:e3000196 pubmed publisher
Calfon M, Zeng H, Urano F, Till J, Hubbard S, Harding H, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature. 2002;415:92-6 pubmed

product information

Catalog Number :

21896

Product Name :

BiP 2: C. elegans BiP (hsp-4)-GFP

article :

doi	10.1038/415092a
id	2771
pubmed_id	11780124

bacterial resistance :

Ampicillin

cloning :

backbone

pPD95.75

backbone_mutation

backbone_origin

backbone_size

4500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Worm Expression

growth notes :

This reporter gene tracks the expression of endogenous BiP (hsp-4) in C. elegans. It consists of a ~1.1 KB genomic C. elegans fragment linked to GFP in the vector pPD95.75. The fusion between the GFP artificial gene and hsp-4 takes place at AA #3 of HSP-4)

growth temp :

Stbl2, 30 degrees

origin :

pi :

alt_names

hsp-4

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	GFP-R
sequencing_primer_5	M13_pUC-rev
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	hsp-4
id	174203

genbank_ids

mutation

Fusion between the GFP and hsp-4 takes place at AA#3 of HSP-4

name

BiP

shRNA_sequence

size

1100

species

6239

Caenorhabditis elegans