This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
boP58-pBabe.puro(notag)
catalog :
21885
citations: 1
Reference
Oyadomari S, Yun C, Fisher E, Kreglinger N, Kreibich G, Oyadomari M, et al. Cotranslocational degradation protects the stressed endoplasmic reticulum from protein overload. Cell. 2006;126:727-39 pubmed
product information
Catalog Number :
21885
Product Name :
boP58-pBabe.puro(notag)
article :
doi10.1016/j.cell.2006.06.051
id2786
pubmed_id16923392
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Addgene sequencing results show there is no HA tag in this plasmid. The start codon of the gag gene has been mutated so that it does not get expressed. Its presence, however,increases the level of retroviral production.
growth temp :
STBL2
origin :
37
pi :
alt_names
DNAJC3
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE-3
sequencing_primer_5pBABE-5
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneDNAJC3
id286770
genbank_ids
mutation
nameP58
shRNA_sequence
size1652
species
tags
locationN terminal on insert
tagHA
resistance markers :
421
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA