pBAD33-mf-lon | Addgene 21867 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBAD33-mf-lon

catalog :

21867

citations: 3

Reference
Szydlo K, Ignatova Z, Gorochowski T. Improving the Robustness of Engineered Bacteria to Nutrient Stress Using Programmed Proteolysis. ACS Synth Biol. 2022;11:1049-1059 pubmed publisher
Maier J, Möhrle R, Jeltsch A. Design of synthetic epigenetic circuits featuring memory effects and reversible switching based on DNA methylation. Nat Commun. 2017;8:15336 pubmed publisher
Gur E, Sauer R. Evolution of the ssrA degradation tag in Mycoplasma: specificity switch to a different protease. Proc Natl Acad Sci U S A. 2008;105:16113-8 pubmed publisher

product information

Catalog Number :

21867

Product Name :

pBAD33-mf-lon

article :

doi	10.1073/pnas.0808802105
id	2781
pubmed_id	18852454

bacterial resistance :

Chloramphenicol

cloning :

backbone

pBAD33

backbone_mutation

The origin is pBR322 not p15a

backbone_origin

Beckwith Lab

backbone_size

5352

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

A detailed protocol for purification of mf-lon can be found in the associated paper.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	BamHI
promoter
sequencing_primer_3	pBAD-rev
sequencing_primer_5	pBAD-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Mfl404
gene	Mfl404
id	2898185

genbank_ids

mutation

all TGA codons changed to TGG codons

name

Lon

shRNA_sequence

size

2326

species

32644

Other

Mesoplasma florum