SREC 1: abu-1::gfp | Addgene 21824 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

SREC 1: abu-1::gfp

catalog :

21824

citations: 1

Reference
Urano F, Calfon M, Yoneda T, Yun C, Kiraly M, Clark S, et al. A survival pathway for Caenorhabditis elegans with a blocked unfolded protein response. J Cell Biol. 2002;158:639-46 pubmed

product information

Catalog Number :

21824

Product Name :

SREC 1: abu-1::gfp

article :

doi	10.1083/jcb.200203086
id	2769
pubmed_id	12186849

bacterial resistance :

Ampicillin

cloning :

backbone

pPD95_75

backbone_mutation

backbone_origin

Available at Addgene (#1494)

backbone_size

4487

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Worm Expression

growth notes :

Addgene sequencing results show several mismatches with author's sequence. abu-1::gfp C. elegans reporter gene. A 1.3-kb fragment of C. elegans genomic DNA immediately 5' of the predicted initiation ATG codon of abu-1 (AC3.3) was amplified by PCR using the oligonucleotides AC3.1S (5'-GGCATTGTGGCACGCATTGAACTG-3') and AC3Bam.2AS (5'-GATAGGATCCATTGTTAATATGCTTGAAGAGCTGC-3') and ligated in frame with the GFP coding region in the plasmid of pPD95.75.

origin :

pi :

alt_names

AC3.3

abu1

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	M13r
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	abu-1
id	181800

genbank_ids

mutation

name

abu-1 promoter

shRNA_sequence

size

1300

species

6239

Caenorhabditis elegans