AIRAP 2: AIRAP(m1.9-T7)pGEX.KG | Addgene 21792 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

AIRAP 2: AIRAP(m1.9-T7)pGEX.KG

catalog :

21792

citations: 1

Reference
Stanhill A, Haynes C, Zhang Y, Min G, Steele M, Kalinina J, et al. An arsenite-inducible 19S regulatory particle-associated protein adapts proteasomes to proteotoxicity. Mol Cell. 2006;23:875-85 pubmed

product information

Catalog Number :

21792

Product Name :

AIRAP 2: AIRAP(m1.9-T7)pGEX.KG

article :

doi	10.1016/j.molcel.2006.07.023
id	2761
pubmed_id	16973439

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX.KG

backbone_mutation

backbone_origin

ATCC

backbone_size

5006

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

We DO NOT have a good record for how this plasmid was constructed. The best guess is that Jin Liu used the m1.9Bam.1S primer and T7 to PCR the CDS + 3'UTR from m1.9pBS and ligated the BamH1-Xba1 fragment in frame with GST in pGEX.KG

growth temp :

Strain should be BL21

inserts :

BL21

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	BamHI
promoter
sequencing_primer_3	pGEX-3
sequencing_primer_5	pGEX-5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Airap, AA407930, AU016206
gene	Zfand2a
id	100494

genbank_ids

AF224494

mutation

name

AIRAP

shRNA_sequence

size

1250

species

10090

Mus musculus