This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pES1002
catalog :
21789
citations: 1
Reference
Yu R, Pesce C, Colman Lerner A, Lok L, Pincus D, Serra E, et al. Negative feedback that improves information transmission in yeast signalling. Nature. 2008;456:755-61 pubmed publisher
product information
Catalog Number :
21789
Product Name :
pES1002
article :
doi10.1038/nature07513
id2760
pubmed_id19079053
bacterial resistance :
Ampicillin
cloning :
backbonepRS406
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
There are several sequence discrepancies between depositor's sequence and Addgene sequence in the 5' UTR. The 5'UTR in the plasmid is from a W303-derived strain, and the SGD used S288C strain for genome sequencing, so the mismatches could be SNPs. This plasmid is functional and these mismatches do not affect the ORF.
origin :
37
pi :
alt_names
Fus3
Fus3-as
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYBL016W, DAC2
geneFUS3
id852265
genbank_ids
NC_001134
mutationcontains 5' 1kb upstream UTR/promoter Q93A mutation in open reading frame 3' 400bp downstream UTR/terminator
nameFus3-as2
shRNA_sequence
size2063
species
4932
Saccharomyces cerevisiae
tags
plasmid copy :
Dr. Eduard Serra (eserra@idibell.org)
resistance markers :
606
tags :
High Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA