This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRY1004
catalog :
21788
citations: 1
Reference
Yu R, Pesce C, Colman Lerner A, Lok L, Pincus D, Serra E, et al. Negative feedback that improves information transmission in yeast signalling. Nature. 2008;456:755-61 pubmed publisher
product information
Catalog Number :
21788
Product Name :
pRY1004
article :
doi10.1038/nature07513
id2760
pubmed_id19079053
bacterial resistance :
Ampicillin
cloning :
backbonepACL7-YFP-A206K
backbone_mutation
backbone_origin
backbone_size3300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Construction was complicated; please refer to parent article's supplemental materials for details of construction.
origin :
37
pi :
alt_names
Dig1
YFP
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL049C, RST1
geneDIG1
id856058
genbank_ids
NC_001148
mutationcontains 5' 1000bp UTR (Dig1 promoter) Dig1 open reading frame YFP 3' ADH terminator from pACL7-YFP-A206K
nameDig1-YFP
shRNA_sequence
size3500
species
4932
Saccharomyces cerevisiae
A. victoria
tags
locationC terminal on insert
tagYFP
resistance markers :
606
tags :
High Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA