This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRY1001
catalog :
21787
citations: 1
Reference
Yu R, Pesce C, Colman Lerner A, Lok L, Pincus D, Serra E, et al. Negative feedback that improves information transmission in yeast signalling. Nature. 2008;456:755-61 pubmed publisher
product information
Catalog Number :
21787
Product Name :
pRY1001
article :
doi10.1038/nature07513
id2760
pubmed_id19079053
bacterial resistance :
Ampicillin
cloning :
backbonepACL7-YFP-A206K
backbone_mutation
backbone_origin
backbone_size5700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Construction was complicated; please refer to parent article's supplemental materials for details of construction. KpnI site is not unique in this plasmid.
origin :
37
pi :
alt_names
CFP
Ste12
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYHR084W
geneSTE12
id856484
genbank_ids
NC_001140
mutationcontains 1000 bp UTR (Ste12 promoter) upstream of CFP-Ste12 ORF contains 3' ADH terminator from pACL7-YFP-A206K.
nameCFP-Ste12
shRNA_sequence
size4254
species
4932
Saccharomyces cerevisiae
A. victoria
tags
locationN terminal on insert
tagCFP
resistance markers :
606
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA