This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RB-409-815-pGex2T
catalog :
21784
citations: 1
Reference
Kaelin W, Pallas D, DeCaprio J, Kaye F, Livingston D. Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product. Cell. 1991;64:521-32 pubmed
product information
Catalog Number :
21784
Product Name :
RB-409-815-pGex2T
article :
doi10.1016/0092-8674(91)90236-R
id2758
pubmed_id1825028
bacterial resistance :
Ampicillin
cloning :
backbonepGex2T
backbone_mutation
backbone_originPharmacia
backbone_size4948
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth temp :
DH5alpha BL21
origin :
37
pi :
alt_names
retinoblastoma protein
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH I
cloning_site_5BamH I
promoter
sequencing_primer_3pGex-3'
sequencing_primer_5pGex-5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110
geneRB1
id5925
genbank_ids
BC040540
mutationdeletion mutant ORF contains aa 409-815 of wt Rb cDNA, contains mutation at codon 341 (full-length Rb aa E748K (GAG to AAG))
nameRb
shRNA_sequence
size1239
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
238
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA