This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Rb-379-928-pGex2TK
catalog :
21749
citations: 1
Reference
Kaelin W, Krek W, Sellers W, DeCaprio J, Ajchenbaum F, Fuchs C, et al. Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties. Cell. 1992;70:351-64 pubmed
product information
Catalog Number :
21749
Product Name :
Rb-379-928-pGex2TK
article :
doi10.1016/0092-8674(92)90108-O
id2720
pubmed_id1638635
bacterial resistance :
Ampicillin
cloning :
backbonepGex2TK
backbone_mutation
backbone_originPharmacia
backbone_size4969
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth temp :
DH5alpha BL21
origin :
37
pi :
alt_names
retinoblastoma protein
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH I
cloning_site_5BamH I
promoter
sequencing_primer_3pGex-3'
sequencing_primer_5pGex-5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110
geneRB1
id5925
genbank_ids
BC040540
mutationdeletion mutant ORF contains aa 379-928 of wt Rb cDNA, contains silent mutation at codon 455 (full-length Rb aa V833 (GTA to GTG)), contains mutation at codon 380 (full-length Rb aa S758L (TCG to TTG))
nameRb
shRNA_sequence
size1670
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
238
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA