This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PAP-delta10-cHIS
catalog :
21716
citations: 1
Reference
Balbo P, Meinke G, Bohm A. Kinetic studies of yeast polyA polymerase indicate an induced fit mechanism for nucleotide specificity. Biochemistry. 2005;44:7777-86 pubmed
product information
Catalog Number :
21716
Product Name :
PAP-delta10-cHIS
article :
doi10.1021/bi050089r
id2732
pubmed_id15909992
bacterial resistance :
Ampicillin
cloning :
backbonePet21b
backbone_mutation
backbone_originEMD Biosciences
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
See attached purification scheme
origin :
37
pi :
alt_names
Pap1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoter
sequencing_primer_3T7-term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYKR002W
genePAP1
id853871
genbank_ids
mutation30 amino acids off the c terminus
namepolyA polymerase
shRNA_sequence
size1500
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagHIS
resistance markers :
43
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA