This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pROSA26-5'
catalog :
21715
citations: 2
Reference
Hsiao E, Nguyen T, Ng J, Scott M, Chang W, Zahed H, et al. Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice. Stem Cell Res Ther. 2011;2:11 pubmed publisher
Soriano P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet. 1999;21:70-1 pubmed
product information
Catalog Number :
21715
Product Name :
pROSA26-5'
article :
doi10.1038/5007
id2734
pubmed_id9916792
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript KS (-)
backbone_mutation
backbone_originStratagene
backbone_size3027
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
for use with mouse targeting vector pROSA26-1
growth notes :
Probe for detecting targeted integrations by Southern blots. It is a small probe so users should wash at low stringency, eg. 1xSSC at 65 degrees. We recommend you order this plasmid along with pROSA26-1 and SAbgeo as a ROSA26 targeting kit . For more information visit - http://research.mssm.edu/soriano/lab
origin :
37
pi :
alt_names
ROSA26
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3T3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGtrgeo26, Gtrosa26, R26, ROSA26, Thumpd3as1
geneGt(ROSA)26Sor
id14910
genbank_ids
mutation
nameROSA26-5' insert
shRNA_sequence
size132
species
10090
Mus musculus
tags
resistance markers :
493
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA